固定化脂肪酶：Covalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil
Covalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil
Recently, PhD student Miss Jing-jing Guo supervised by Prof. Zhen Fang published a research article in Bioresource Technology about preparing immobilized lipase for biodiesel production from high acid value oil.
Magnetic biochar was synthesized via chelation of Fe3+ with carboxymethyl cellulose and pyrolysis for covalently immobilizing Eversa® Transform lipase. The magnetic biochar had 75.8 mg/g lipase loading that was 54.1% higher than that without magnetism. The immobilized lipase achieved at 91.3 mg/g lipase loading with 19.2 U/mg lipase activity after optimized. It showed good thermal and acid stability with 82.5% and 98.2% relative activity at 45 °C and pH 4, respectively. Its relative activity was 90.8% after stored for 30 d at 4 °C. After magnetically separated for 10 cycles, it still kept 70.1% activity due to the strong covalent bonding. The lipase further catalyzed one-pot esterification and transesterification of high acid value oil (38 mg KOH/g) with 95.7% biodiesel yield and cycled for 10 times at 85.7% yield. Kinetic study gave the activation energy of 28.7 kJ/mol. The covalently immobilized lipase could find practical applications.
Related results were accepted in Bioresource Technology:
JJ Guo, YT Wang, Zhen Fang* (Supervisor). Covalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil. Bioresource Technology, 394, 130237 (2024). https://doi.org/10.1016/j.biortech.2023.130237.
The immobilized lipase catalyzed high acid value oil for biodiesel production with 95.7% yield and recycled for 10 times at 85.7% yield. （固定化脂肪酶催化高酸值油制备生物柴油的产率为95.7%。酶回收10次，产率仍可达到85.7%。）
磁性生物炭共价固定化脂肪酶一步法从高酸值油中制备生物柴油
最近，博士生郭静静在方真教授的指导下，在国际学术期刊Bioresource Technology （Q1，IF 11.4）发表一篇关于使用共价固定化脂肪酶从高酸值油中制备生物柴油的研究性论文。
通过Fe3+与羧甲基纤维素螯合热解制备了磁性生物炭，用于脂肪酶Eversa® Transform的共价固定化。磁性生物炭的酶载量为75.8 mg/g，比非磁性生物炭提高了54.1%。经优化后，固定化脂肪酶的酶载量为91.3 mg/g，酶活为19.2 U/mg。在45 °C和pH 4条件下，固定化酶的相对活性分别为82.5%和98.2%，表现出良好的热稳定性和耐酸性。在4 °C贮藏30天后，该酶的相对活性为90.8%。通过磁分离使用10次后，由于强的共价键，该酶仍保留了70.1%的相对活性。固定化脂肪酶催化高酸值油制备生物柴油的产率为95.7%，该酶回收10次，生物柴油产率仍可达到85.7%。动力学研究表明该反应的活化能为28.7 kJ/mol。该共价固定化脂肪酶具有实际应用价值。
JJ Guo, YT Wang, Zhen Fang* (Supervisor). Covalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil. Bioresource Technology, 394, 130237 (2024). https://doi.org/10.1016/j.biortech.2023.130237.