Complete recovery of cellulose from rice straw pretreated with EG-AlCl3 for enzymatic hydrolysis
Ethylene glycol (EG) and AlCl3 pretreated rice straw at 150 °C with 0.055 mol/L AlCl3 for 0.5 h, resulting in 100% cellulose recovery with 88% lignin and 90% hemicellulose removal for a remarkable glucose yield of 94% after 24 h enzymatic hydrolysis. (在150 °C和0.055 mol/L AlCl3条件下，水稻秸秆经乙二醇-水预处理0.5 h后，保留100%的纤维素，并去除88%的木质素和90%的半纤维素；10 FPU/g和5%基质浓度条件下，酶水解24 h后，预处理后水稻秸秆的酶解率达到94%。)
Recently, PhD student Mr. Song Tang supervised by Prof. Zhen FANG developed a novel ethylene glycol (EG) and aluminum chloride pretreatment for lignocellulosic biomass. EG-AlCl3 pretreatment had an extremely good selectivity for component fractionation, resulting in 88% delignification and 90% hemicellulose removal, with 100% cellulose recovered or 76% (w/w) cellulose content in solid residue at 150 °C with 0.055 mol/L AlCl3. The pretreated residue (5%, w/v) presented a higher enzymatic hydrolysis rate (glucose yield increased 2 times to 94%) for 24 h at cellulase loading of 10 FPU/g. The hydrolysis behavior was correlated with the composition and structure of substrates characterized by SEM, FT-IR, BET, XRD and TGA. The enzyme adsorption ability of pretreated straw was 12-folds that for the original sample. EG-AlCl3 solution was further cycled for 3 times with 100% cellulose recovery but only 29% lignin removal due to the loss of AlCl3.
The results were published:
S Tang, Q Dong, Zhen Fang*, ZD Miao, Complete Recovery of Cellulose from Rice Straw Pretreated with Ethylene Glycol and Aluminum Chloride for Enzymatic Hydrolysis, Bioresource Technology, 284, 98–104(2019). https://doi.org/10.1016/j.biortech.2019.03.100.
最近，博士生唐松（男）同学在方老师的指导下，开发出一种新型的应用于木质纤维素生物质的乙二醇-氯化铝预处理技术，其对木质纤维素组分分离具有极好的选择性。在150 °C和0.055 mol/L AlCl3条件下，水稻秸秆中纤维素被100%保留，88%的木质素和90%的半纤维素被去除。同时，固体残渣中纤维素含量达到76%，且在10 FPU/g和5%基质浓度条件下，酶水解24 h后，酶解率较原始水稻秸秆提高2倍，达到了94%。通过SEM、FT-IR、BET、XRD和TGA等技术手段表征了水稻秸秆的组成结构和其可酶水解能力的关系。水稻秸秆经预处理后，对纤维素酶的吸附能力提高了11倍。此外，乙二醇-氯化铝溶液用于水稻秸秆循环处理3次，均保持100%纤维素回收率，但由于循环处理期间AlCl3的损失，木质素脱除率降至29%。
结果发表在Bioresource Technology: S Tang, Q Dong, Zhen Fang*, ZD Miao, Complete Recovery of Cellulose from Rice Straw Pretreated with Ethylene Glycol and Aluminum Chloride for Enzymatic Hydrolysis, Bioresource Technology, 284, 98–104 (2019). https://doi.org/10.1016/j.biortech.2019.03.100 (Q1, IF 5.8).