{"id":5881,"date":"2026-01-10T18:06:03","date_gmt":"2026-01-10T10:06:03","guid":{"rendered":"https:\/\/woodrefinery.com\/zhenfang\/?p=5881"},"modified":"2026-01-10T18:06:03","modified_gmt":"2026-01-10T10:06:03","slug":"%e5%be%ae%e7%94%9f%e7%89%a9%e6%b2%b9%e8%84%82efficient-lignin-removal-from-rice-straw-via-alkaline-freeze-combined-with-hydrothermal-pretreatment-to-obtain-high-titer-sugar-for-microbial-lipid-product","status":"publish","type":"post","link":"https:\/\/woodrefinery.com\/zhenfang\/%e5%be%ae%e7%94%9f%e7%89%a9%e6%b2%b9%e8%84%82efficient-lignin-removal-from-rice-straw-via-alkaline-freeze-combined-with-hydrothermal-pretreatment-to-obtain-high-titer-sugar-for-microbial-lipid-product\/","title":{"rendered":"\u5fae\u751f\u7269\u6cb9\u8102Efficient lignin removal from rice straw via alkaline-freeze combined with hydrothermal pretreatment to obtain high-titer sugar for microbial lipid production"},"content":{"rendered":"<p style=\"text-align: center;\"><strong>Efficient lignin removal from rice straw via alkaline-freeze combined with hydrothermal pretreatment to obtain high-titer sugar for microbial lipid production<\/strong><\/p>\n<p style=\"text-align: justify;\">Recently, PhD student Miss Dong Qian supervised by Prof. <strong>Zhen Fang<\/strong> published a research article in <em>Biomass and Bioenergy<\/em> (Q2, Impact Factor: 5.8) about microbial lipid production from rice straw.<\/p>\n<p style=\"text-align: justify;\">This study developed an integrated process for converting rice straw into microbial lipids. The straw was first pretreated using alkali\u2011freeze combined with hydrothermal method, followed by high\u2011solid enzymatic hydrolysis to produce fermentable sugars for lipid fermentation. Alkali\u2011freeze combined with hydrothermal pretreatment had an extremely good selectivity for component fractionation, resulting in 86.9% delignification, and 97.1% cellulose recovery, 78.8% hemicellulose recovery. After hydrolysis, glucose and xylose yield of pretreated straw reached 91.1% and 68.9%, respectively. Further optimization with response surface methodology increased the yields to 100% for glucose and 81.1% for xylose. Fed\u2011batch saccharification was applied under low enzyme loading (10\u202fFPU\/g cellulase, 20% solid loading), producing 111.4\u202fg\/L glucose and 36.3\u202fg\/L xylose, with corresponding yields of 83.4% and 66.3%. Using the mixed sugars, cultivation conditions for <em>Cryptococcus curvatus<\/em> were optimized, achieving a lipid concentration of 6\u202fg\/L and a yield of 0.175\u202fg\/g. Adding Tween\u202f80 increased these to 7.7\u202fg\/L and 0.21\u202fg\/g, respectively. Finally, the hydrolysates were separated for lipid fermentation, producing 7.3\u202fg\/L lipids with a yield of 0.198\u202fg\/g. This work provides a promising strategy for the conversion and utilization of agricultural straw.<\/p>\n<p style=\"text-align: justify;\">Related results were accepted in<em> Biomass and Bioenergy<\/em>\uff1a<\/p>\n<p style=\"text-align: justify;\">Q Dong, S Tang*,<strong> Zhen Fang*<\/strong>\uff0cEfficient lignin removal from rice straw via alkaline-freeze combined with hydrothermal pretreatment to obtain high-titer sugar for microbial lipid production. Biomass and Bioenergy <strong>2026, 209<\/strong>, 108933. <a href=\"https:\/\/doi.org\/10.1016\/j.biombioe.2026.108933\">https:\/\/doi.org\/10.1016\/j.biombioe.2026.108933<\/a><\/p>\n<p style=\"text-align: justify;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5882\" src=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2026\/01\/5-Graphical-Abstract.png\" alt=\"\" width=\"905\" height=\"530\" srcset=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2026\/01\/5-Graphical-Abstract.png 905w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2026\/01\/5-Graphical-Abstract-300x176.png 300w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2026\/01\/5-Graphical-Abstract-768x450.png 768w\" sizes=\"(max-width: 905px) 100vw, 905px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>Microbial lipid production from pretreated rice straw hydrolysates (<\/strong><strong>\u6c34\u7a3b\u79f8\u79c6\u7ecf\u78b1\u51b7\u51bb\u7ed3\u5408\u6c34\u70ed\u9884\u5904\u7406\u540e\uff0c\u5206\u6279\u8865\u6599\u9176\u89e3\uff0c\u5c06\u6c34\u89e3\u4ea7\u7269\u4f5c\u4e3a\u78b3\u6e90\uff0c\u901a\u8fc7\u5f2f\u66f2\u9690\u7403\u83cc\u9ad8\u6548\u751f\u4ea7\u6cb9\u8102<\/strong><strong>)<\/strong><\/p>\n<hr \/>\n<p style=\"text-align: center;\"><strong>\u6c34\u7a3b\u79f8\u79c6\u7ecf\u78b1\u51b7\u51bb\u7ed3\u5408\u6c34\u70ed\u9884\u5904\u7406\u540e\uff0c\u901a\u8fc7\u5f2f\u66f2\u9690\u7403\u83cc\u9ad8\u6548\u751f\u4ea7\u6cb9\u8102<\/strong><\/p>\n<p style=\"text-align: justify;\">\u6700\u8fd1\uff0c\u535a\u58eb\u751f\u8463\u5029\u5728\u65b9\u771f\u6559\u6388\u7684\u6307\u5bfc\u4e0b\uff0c\u5728\u56fd\u9645\u5b66\u672f\u671f\u520a<em>Biomass and Bioenergy<\/em> (Q2; Impact factor: 5.8)\u4e0a\u53d1\u8868\u4e86\u4e00\u7bc7\u5173\u4e8e\u5229\u7528\u6c34\u7a3b\u79f8\u79c6\u4ea7\u5fae\u751f\u7269\u6cb9\u8102\u7684\u7814\u7a76\u6027\u8bba\u6587\u3002<\/p>\n<p style=\"text-align: justify;\">\u901a\u8fc7\u78b1\u51b7\u51bb\u7ed3\u5408\u6c34\u70ed\u9884\u5904\u7406\u548c\u9ad8\u56fa\u8f7d\u9176\u89e3\uff0c\u4ece\u6c34\u7a3b\u79f8\u79c6\u4e2d\u83b7\u53d6\u9ad8\u6d53\u5ea6\u53ef\u53d1\u9175\u6027\u7cd6\uff0c\u4e3a\u5fae\u751f\u7269\u6cb9\u8102\u53d1\u9175\u63d0\u9ad8\u4f18\u8d28\u78b3\u6e90\u3002\u9996\u5148\uff0c\u901a\u8fc7\u5355\u56e0\u7d20\u5b9e\u9a8c\u786e\u5b9a\u6700\u4f73\u9884\u5904\u7406\u6761\u4ef6\uff0c\u5728\u6b64\u6761\u4ef6\u4e0b\uff0c\u7ea4\u7ef4\u7d20\u548c\u534a\u7ea4\u7ef4\u7d20\u6536\u7387\u5206\u522b\u4e3a97.1%\u548c78.8%\uff0c\u6728\u8d28\u7d20\u53bb\u9664\u7387\u4e3a86.9%\u3002\u9884\u5904\u7406\u79f8\u79c6\u7ecf\u9176\u89e3\u540e\uff0c\u8461\u8404\u7cd6\u548c\u6728\u7cd6\u4ea7\u7387\u5206\u522b\u8fbe\u523091.1%\u548c68.9%\u3002\u968f\u540e\uff0c\u901a\u8fc7\u54cd\u5e94\u9762\u4f18\u5316\u9176\u89e3\u6761\u4ef6\uff0c\u8461\u8404\u7cd6\u548c\u6728\u7cd6\u4ea7\u7387\u5206\u522b\u63d0\u9ad8\u81f3100%\u548c81.1%\u3002\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u901a\u8fc7\u5206\u6279\u8865\u6599\u5b9e\u73b0\u9884\u5904\u7406\u79f8\u79c6\u5728\u4f4e\u9176\u8f7d\u91cf\u4e0b\u7684\u9ad8\u6d53\u7cd6\u5316 (10 FPU\/g\u7ea4\u7ef4\u7d20\u9176\uff0c20%\u56fa\u8f7d\u91cf)\uff0c\u83b7\u5f97111.4 g\/L\u8461\u8404\u7cd6\u548c36.3 g\/L\u6728\u7cd6\uff0c\u5bf9\u5e94\u4ea7\u7387\u5206\u522b\u4e3a83.4%\u548c66.3%\u3002\u6700\u540e\uff0c\u901a\u8fc7\u5355\u56e0\u7d20\u5b9e\u9a8c\u786e\u5b9a\u5f2f\u66f2\u9690\u7403\u83cc\u53d1\u9175\u6df7\u7cd6 (\u8461\u8404\u7cd6\u548c\u6728\u7cd6) \u4ea7\u6cb9\u8102\u6761\u4ef6\uff0c\u5728\u6700\u4f73\u6761\u4ef6\u4e0b\uff0c\u603b\u6cb9\u8102\u91cf\u4e3a6 g\/L\uff0c\u6cb9\u8102\u4ea7\u7387\u8fbe\u52300.175 g\/g\uff0c\u5728\u57f9\u517b\u57fa\u4e2d\u6dfb\u52a0\u5410\u6e2980\uff0c\u603b\u6cb9\u8102\u91cf\u548c\u6cb9\u8102\u4ea7\u7387\u5206\u522b\u63d0\u9ad8\u81f37.7 g\/L\u548c0.21 g\/L\u3002\u57fa\u4e8e\u8be5\u57f9\u517b\u6761\u4ef6\uff0c\u5c06\u9884\u5904\u7406\u79f8\u79c6\u6c34\u89e3\u4ea7\u7269\u4f5c\u4e3a\u78b3\u6e90\u57f9\u517b\u6cb9\u8102\u9175\u6bcd\uff0c\u4ea7\u51fa\u6cb9\u81027.3 g\/L\uff0c\u5176\u4ea7\u7387\u8fbe\u52300.198 g\/g\u3002\u672c\u7814\u7a76\u4e3a\u519c\u4f5c\u7269\u79f8\u79c6\u7684\u8f6c\u5316\u5229\u7528\u63d0\u4f9b\u4e86\u65b0\u601d\u8def\u3002<\/p>\n<p style=\"text-align: justify;\">\u7ed3\u679c\u53d1\u8868\u5728<em>Biomass and Bioenergy<\/em>\uff1a<\/p>\n<p style=\"text-align: justify;\">Q Dong, S Tang*,<strong> Zhen Fang*<\/strong>\uff0cEfficient lignin removal from rice straw via alkaline-freeze combined with hydrothermal pretreatment to obtain high-titer sugar for microbial lipid production. Biomass and Bioenergy <strong>2026, 209<\/strong>, 108933. <a href=\"https:\/\/doi.org\/10.1016\/j.biombioe.2026.108933\">https:\/\/doi.org\/10.1016\/j.biombioe.2026.108933<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Efficient lignin removal from rice straw via alkaline-f [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5881"}],"collection":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/comments?post=5881"}],"version-history":[{"count":1,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5881\/revisions"}],"predecessor-version":[{"id":5883,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5881\/revisions\/5883"}],"wp:attachment":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/media?parent=5881"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/categories?post=5881"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/tags?post=5881"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}