{"id":5353,"date":"2024-12-08T16:36:25","date_gmt":"2024-12-08T08:36:25","guid":{"rendered":"https:\/\/woodrefinery.com\/zhenfang\/?p=5353"},"modified":"2024-12-08T17:04:50","modified_gmt":"2024-12-08T09:04:50","slug":"%e7%a3%81%e6%80%a7%e7%96%8f%e6%b0%b4%e7%94%9f%e7%89%a9%e7%82%ad%e5%9b%ba%e5%ae%9a%e5%8c%96%e8%84%82%e8%82%aa%e9%85%b6%ef%bc%9adual-lipases-immobilized-on-magnetic-hydrophobic-biochar-for-one-step-prod","status":"publish","type":"post","link":"https:\/\/woodrefinery.com\/zhenfang\/%e7%a3%81%e6%80%a7%e7%96%8f%e6%b0%b4%e7%94%9f%e7%89%a9%e7%82%ad%e5%9b%ba%e5%ae%9a%e5%8c%96%e8%84%82%e8%82%aa%e9%85%b6%ef%bc%9adual-lipases-immobilized-on-magnetic-hydrophobic-biochar-for-one-step-prod\/","title":{"rendered":"\u78c1\u6027\u758f\u6c34\u751f\u7269\u70ad\u56fa\u5b9a\u5316\u8102\u80aa\u9176\uff1aDual lipases immobilized on magnetic hydrophobic biochar for one-step production of biodiesel in deep eutectic solvent"},"content":{"rendered":"

Dual lipases immobilized on magnetic hydrophobic biochar for one-step production of biodiesel in deep eutectic solvent<\/p>\n

Recently, PhD student Miss Jing-jing Guo supervised by Prof. Zhen Fang<\/strong> published a research article in Fuel about biodiesel production from high acid value oil with immobilized dual lipases.<\/p>\n

Magnetic hydrophobic biochar was synthesized via <\/em>pyrolysis of the chelated carboxymethyl cellulose\/Fe3+<\/sup> and modification by composite silanes, and subsequently used to covalently\/physically immobilize dual lipases CALB\/ET. The activity of dual lipases immobilized on hydrophobic magnetic biochar (16.1 U\/mg) was increased by 11.8% than magnetic biochar, or 3.9% and 8.1% higher than that of single immobilized lipase ET (15.5 U\/mg) and immobilized lipase CALB (14.9 U\/mg), respectively. The highest loading of 39.5 mg lipase\/g support was obtained with 17.9 U\/mg activity. The immobilized dual lipases were stable with relative activity of 92.6% and 95.4% at 50 \u00b0C and pH<\/em> 9 vs.<\/em> 74.6% and 83.9% for liquid dual lipases, respectively. The immobilized dual lipases were further used for one-step production of biodiesel from acid oil (acid value 38 mg KOH\/g) in deep eutectic solvent at 97.9% yield with 5 cycles (75.2%). Enzymatic reactions promote biodiesel purification, and crude biodiesel had 0.27 mg KOH\/g acid value and 0.015% glycerol that met both Chinese national and US ASTM standards. The activation energy was 41.2 kJ\/mol with kinetic Michaelis constant of 7.92\u00d710-1<\/sup> mol\/L. The immobilized dual lipases for one-step production and purification of biodiesel presented excellent activity that provided a new insight for the development of novel immobilized lipase in biodiesel production.<\/p>\n

Related results were published in Fuel:<\/p>\n

Jing-jing Guo, Li Wang, Yi-tong Wang, Wo-na Ding, Wei-wei Liu, Zhen Fang*<\/strong>. Dual lipases immobilized on magnetic hydrophobic biochar for one-step production of biodiesel in deep eutectic solvent. Fuel (IF 6.7), 381 (2025)<\/strong>, 133497. https:\/\/doi.org\/10.1016\/j.fuel.2024.133497<\/a><\/p>\n

\"\"<\/p>\n

Two lipases were successfully covalent\/physical immobilized on magnetic hydrophobic biochar for direct production of biodiesel at 97.9% yield from acid oil at low temperature of 35 \u00b0C. \uff08\u53cc\u8102\u80aa\u9176\u6210\u529f\u5171\u4ef7\/\u7269\u7406\u56fa\u5b9a\u5316\u5728\u78c1\u6027\u758f\u6c34\u751f\u7269\u70ad\u4e0a\u5e76\u7528\u4e8e\u572835 \u00b0C\u4f4e\u6e29\u4e0b\u50ac\u5316\u9178\u6027\u6cb9\u5236\u5907\u751f\u7269\u67f4\u6cb9\uff0c\u4ea7\u7387\u4e3a97.9%\uff09\u3002<\/p>\n

\n

\u78c1\u6027\u758f\u6c34\u751f\u7269\u70ad\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176\u5728\u4f4e\u5171\u7194\u6eb6\u5242\u4e2d\u4e00\u6b65\u6cd5\u751f\u4ea7\u751f\u7269\u67f4\u6cb9<\/strong><\/p>\n

\u8fd1\u671f\uff0c\u535a\u58eb\u751f\u90ed\u9759\u9759\u540c\u5b66\u5728\u65b9\u771f\u6559\u6388<\/strong>\u7684\u6307\u5bfc\u4e0b\uff0c\u5728\u56fd\u9645\u5b66\u672f\u671f\u520aFuel\uff08Q1\uff0cIF 6.7\uff09\u53d1\u8868\u4e86\u4e00\u7bc7\u5173\u4e8e\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176\u50ac\u5316\u9ad8\u9178\u503c\u6cb9\u5236\u5907\u751f\u7269\u67f4\u6cb9\u7684\u7814\u7a76\u6027\u8bba\u6587\u3002<\/p>\n

\u901a\u8fc7\u70ed\u89e3\u7fa7\u7532\u57fa\u7ea4\u7ef4\u7d20\/Fe3+<\/sup>\u87af\u5408\u7269\u548c\u590d\u5408\u7845\u70f7\u7684\u6539\u6027\u5236\u5907\u4e86\u78c1\u6027\u758f\u6c34\u751f\u7269\u70ad\uff0c\u5e76\u7528\u4e8e\u5171\u4ef7\/\u7269\u7406\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176CALB\/ET\u3002\u53cc\u8102\u80aa\u9176\u56fa\u5b9a\u5316\u5728\u758f\u6c34\u78c1\u6027\u751f\u7269\u70ad\u4e0a\u7684\u6d3b\u6027\uff0816.1 U\/mg\uff09\u6bd4\u5176\u56fa\u5b9a\u5316\u5728\u78c1\u6027\u751f\u7269\u70ad\u4e0a\u63d0\u9ad8\u4e8611.8%\u3002\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176CALB\/ET\u7684\u6d3b\u6027\u6bd4\u56fa\u5b9a\u5316\u8102\u80aa\u9176ET\uff0815.5 U\/mg\uff09\u548c\u56fa\u5b9a\u5316\u8102\u80aa\u9176CALB\uff0814.9 U\/mg\uff09\u5206\u522b\u63d0\u9ad8\u4e863.9%\u548c8.1%\u3002\u6700\u9ad8\u9176\u8f7d\u91cf\u4e3a39.5 mg\u8102\u80aa\u9176\/g\u8f7d\u4f53\uff0c\u9176\u6d3b\u4e3a17.9 U\/mg\u3002\u572850 \u00b0C\u548cpH<\/em> 9\u65f6\uff0c\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176\u7684\u76f8\u5bf9\u9176\u6d3b\u5206\u522b\u4e3a92.6%\u548c95.4%\uff0c\u800c\u6db2\u4f53\u53cc\u8102\u80aa\u9176\u7684\u76f8\u5bf9\u9176\u6d3b\u4e3a74.6%\u548c83.9%\u3002\u5728\u4f4e\u5171\u7194\u6eb6\u5242\u4f53\u7cfb\u4e2d\uff0c\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176\u7528\u4e8e\u50ac\u5316\u9178\u6027\u6cb9\uff08\u9178\u503c38 mg KOH\/g\uff09\u4e00\u6b65\u6cd5\u5236\u5907\u751f\u7269\u67f4\u6cb9\uff0c\u4ea7\u7387\u4e3a97.9%\u3002\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176\u5faa\u73af5\u6b21\uff0c\u751f\u7269\u67f4\u6cb9\u4ea7\u7387\u4ecd\u4e3a75.2%\u3002\u8be5\u53cd\u5e94\u4f53\u7cfb\u4fc3\u8fdb\u4e86\u751f\u7269\u67f4\u6cb9\u7684\u7eaf\u5316\uff0c\u7c97\u751f\u7269\u67f4\u6cb9\u7684\u9178\u503c\u4e3a0.27 mg KOH\/g\uff0c\u7518\u6cb9\u542b\u91cf\u4e3a0.015%\uff0c\u7b26\u5408\u4e2d\u56fd\u548c\u7f8e\u56fdASTM\u7684\u6807\u51c6\u3002\u52a8\u529b\u5b66\u7814\u7a76\u8868\u660e\u8be5\u53cd\u5e94\u7684\u6d3b\u5316\u80fd\u4e3a41.2 kJ\/mol\uff0c\u52a8\u529b\u5b66\u7c73\u6c0f\u5e38\u6570\u4e3a7.92\u00d710-1<\/sup> mol\/L\u3002\u56fa\u5b9a\u5316\u53cc\u8102\u80aa\u9176\u5728\u4e00\u6b65\u6cd5\u751f\u4ea7\u548c\u7eaf\u5316\u751f\u7269\u67f4\u6cb9\u4e2d\u8868\u73b0\u51fa\u4f18\u5f02\u7684\u6d3b\u6027\uff0c\u4e3a\u751f\u7269\u67f4\u6cb9\u751f\u4ea7\u4e2d\u65b0\u578b\u56fa\u5b9a\u5316\u8102\u80aa\u9176\u7684\u5f00\u53d1\u63d0\u4f9b\u4e86\u65b0\u7684\u89c1\u89e3\u3002<\/p>\n

\u7ed3\u679c\u53d1\u8868\u5728Fuel:<\/p>\n

Jing-jing Guo, Li Wang, Yi-tong Wang, Wo-na Ding, Wei-wei Liu, Zhen Fang*<\/strong>. Dual lipases immobilized on magnetic hydrophobic biochar for one-step production of biodiesel in deep eutectic solvent. Fuel (IF 6.7), 381 (2025)<\/strong>, 133497. https:\/\/doi.org\/10.1016\/j.fuel.2024.133497<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Dual lipases immobilized on magnetic hydrophobic biocha […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5353"}],"collection":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/comments?post=5353"}],"version-history":[{"count":2,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5353\/revisions"}],"predecessor-version":[{"id":5361,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5353\/revisions\/5361"}],"wp:attachment":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/media?parent=5353"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/categories?post=5353"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/tags?post=5353"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}