{"id":5249,"date":"2024-02-29T19:46:07","date_gmt":"2024-02-29T11:46:07","guid":{"rendered":"https:\/\/woodrefinery.com\/zhenfang\/?p=5249"},"modified":"2024-03-15T18:02:26","modified_gmt":"2024-03-15T10:02:26","slug":"%e5%9b%ba%e5%ae%9a%e5%8c%96%e8%84%82%e8%82%aa%e9%85%b6%ef%bc%9acovalent-immobilization-of-lipase-on-magnetic-biochar-for-one-pot-production-of-biodiesel-from-high-acid-value-oil-2","status":"publish","type":"post","link":"https:\/\/woodrefinery.com\/zhenfang\/%e5%9b%ba%e5%ae%9a%e5%8c%96%e8%84%82%e8%82%aa%e9%85%b6%ef%bc%9acovalent-immobilization-of-lipase-on-magnetic-biochar-for-one-pot-production-of-biodiesel-from-high-acid-value-oil-2\/","title":{"rendered":"\u56fa\u5b9a\u5316\u8102\u80aa\u9176\uff1aCovalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil"},"content":{"rendered":"

Covalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil<\/strong><\/p>\n

Recently, PhD student Miss Jing-jing Guo supervised by Prof. Zhen Fang<\/strong> published a research article in Bioresource Technology about preparing immobilized lipase for biodiesel production from high acid value oil.<\/p>\n

Magnetic biochar was synthesized via<\/em> chelation of Fe3+<\/sup> with carboxymethyl cellulose and pyrolysis for covalently immobilizing Eversa\u00ae Transform lipase. The magnetic biochar had 75.8 mg\/g lipase loading that was 54.1% higher than that without magnetism. The immobilized lipase achieved at 91.3 mg\/g lipase loading with 19.2 U\/mg lipase activity after optimized. It showed good thermal and acid stability with 82.5% and 98.2% relative activity at 45 \u00b0C and pH<\/em> 4, respectively. Its relative activity was 90.8% after stored for 30 d at 4 \u00b0C. After magnetically separated for 10 cycles, it still kept 70.1% activity due to the strong covalent bonding. The lipase further catalyzed one-pot esterification and transesterification of high acid value oil (38 mg KOH\/g) with 95.7% biodiesel yield and cycled for 10 times at 85.7% yield. Kinetic study gave the activation energy of 28.7 kJ\/mol. The covalently immobilized lipase could find practical applications.<\/p>\n

Related results were accepted in Bioresource Technology:<\/p>\n

JJ Guo, YT Wang, Zhen Fang* (Supervisor). Covalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil. Bioresource Technology, 394, 130237 (2024<\/strong>).\u00a0 https:\/\/doi.org\/10.1016\/j.biortech.2023.130237<\/a>.<\/p>\n

\"\"<\/p>\n

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\u78c1\u6027\u751f\u7269\u70ad\u5171\u4ef7\u56fa\u5b9a\u5316\u8102\u80aa\u9176\u4e00\u6b65\u6cd5\u4ece\u9ad8\u9178\u503c\u6cb9\u4e2d\u5236\u5907\u751f\u7269\u67f4\u6cb9<\/strong><\/p>\n

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JJ Guo, YT Wang, Zhen Fang* (Supervisor). Covalent immobilization of lipase on magnetic biochar for one-pot production of biodiesel from high acid value oil. Bioresource Technology, 394, 130237 (2024).\u00a0 https:\/\/doi.org\/10.1016\/j.biortech.2023.130237<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

Covalent immobilization of lipase on magnetic biochar f […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5249"}],"collection":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/comments?post=5249"}],"version-history":[{"count":2,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5249\/revisions"}],"predecessor-version":[{"id":5255,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5249\/revisions\/5255"}],"wp:attachment":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/media?parent=5249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/categories?post=5249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/tags?post=5249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}