{"id":5142,"date":"2023-08-26T18:59:26","date_gmt":"2023-08-26T10:59:26","guid":{"rendered":"http:\/\/woodrefinery.com\/zhenfang\/?p=5142"},"modified":"2023-08-26T19:01:06","modified_gmt":"2023-08-26T11:01:06","slug":"%e5%be%ae%e7%94%9f%e7%89%a9%e6%b2%b9%e8%84%82production-of-microbial-lipid-from-food-wastes-with-cryptococcus-curvatus","status":"publish","type":"post","link":"https:\/\/woodrefinery.com\/zhenfang\/%e5%be%ae%e7%94%9f%e7%89%a9%e6%b2%b9%e8%84%82production-of-microbial-lipid-from-food-wastes-with-cryptococcus-curvatus\/","title":{"rendered":"\u5fae\u751f\u7269\u6cb9\u8102Production of microbial lipid from food wastes with Cryptococcus curvatus<\/em>"},"content":{"rendered":"

Two-stage process production of microbial lipid by co-fermentation of glucose and N<\/em>-acetylglucosamine from food wastes with Cryptococcus curvatus<\/em><\/strong><\/p>\n

Recently, master student Mr. Jia-xuan Zhang supervised by Prof. Zhen Fang<\/strong> published a research article in Bioresource Technology about Two-stage fermentation of food waste (rice and shrimp shells hydrolysates) by Cryptococcus curvatus<\/em> for lipids.<\/p>\n

Microbial lipids were produced through a two-stage process with Cryptococcus curvatus<\/em> by co-fermenting rice and shrimp shells hydrolysates. The main components of the lipid sample were long-chain fatty acids with carbon chain lengths of 16 and 18, which were similar to those of typical vegetable oils. And the lipid sample showed high potential for biodiesel production. In the shake flask fermentation, co-utilization of N<\/em>-acetylglucosamine (GlcNAc) and glucose resulted in improved cell mass and lipid production. The highest cell concentration of 17.60 g\/L was achieved in the first-stage, and the highest lipid yield was 0.233 g\/g in the second- stage. Scaling up to a 5-L bioreactor increased lipid content to 60.07 % with 0.233 g\/g yield. When Cryptococcus curvatus<\/em> was cultured in the blends of rice hydrolysates and shrimp shells hydrolysate, lipid content and yield were 52.25 % and 0.204 g\/g. Co-fermentation of rice hydrolysates and chitin hydrolysate is an effective means for recycling food wastes for lipids.<\/p>\n

Related results were published in Bioresource Technology:<\/p>\n

JX Zhang, XL Liu, L Wang, Zhen Fang<\/strong>*, Two-stage process production of microbial lipid by co-fermentation of glucose and N-acetylglucosamine from food wastes with Cryptococcus curvatus<\/em>, Bioresource Technology, 387, 129685, https:\/\/doi.org\/10,1016\/j.biortech.2023.129685<\/a> (2023<\/strong>).<\/p>\n

\"\"\"\"<\/a>\"\"Two-stage process production of microbial lipid by co-fermentation with rice and shrimp shells hydrolysates from food wastes was carried out. After 36 h of the first-stage fermentation, 12.51 g\/L of glucose and 9.91 g\/L of GlcNAc were consumed simultaneously with 12.18 g\/L cell mass produced. Transferring 6 g\/L of yeast cells to the second-stage fermentation medium, cell mass of 19.44 g\/L, lipid content of 52.25 %, and lipid yield of 0.204 g\/g were obtained, respectively. (\u4ee5\u98df\u54c1\u5e9f\u5f03\u7269\u4e2d\u7684\u5927\u7c73\u6c34\u89e3\u7269\u548c\u867e\u58f3\u7684\u6c34\u89e3\u7269\u4e3a\u539f\u6599\uff0c\u91c7\u7528\u4e24\u9636\u6bb5\u5171\u53d1\u9175\u5de5\u827a\u751f\u4ea7\u5fae\u751f\u7269\u6cb9\u8102\u3002\u5728\u7b2c\u4e00\u9636\u6bb5\u53d1\u917536 h\u540e\uff0c12.51 g\/L \u8461\u8404\u7cd6\u548c9.91 g\/L N<\/em> -\u4e59\u9170\u6c28\u57fa\u8461\u8404\u7cd6\u88ab\u6d88\u8017\uff0c\u7ec6\u80de\u4ea7\u91cf\u4e3a12.18 g\/L\u3002\u7136\u540e\u5c066g\/L\u7684\u9175\u6bcd\u7ec6\u80de\u8f6c\u79fb\u5230\u7b2c\u4e8c\u9636\u6bb5\u53d1\u9175\u57f9\u517b\u57fa\u4e2d\u3002\u5206\u522b\u83b7\u5f9719.44g\/L\u7684\u7ec6\u80de\u8d28\u91cf\u300152.25%\u7684\u8102\u8d28\u542b\u91cf\u548c0.204g\/g\u7684\u8102\u8d28\u4ea7\u7387\u3002<\/p>\n

\n

Cryptococcus curvatus<\/em><\/strong>\u5171\u53d1\u9175\u98df\u54c1\u5e9f\u5f03\u7269\u4e2d\u8461\u8404\u7cd6\u548c<\/strong>N<\/em><\/strong> –<\/strong>\u4e59\u9170\u6c28\u57fa\u8461\u8404\u7cd6\u4e24\u9636\u6bb5\u5de5\u827a\u751f\u4ea7\u5fae\u751f\u7269\u8102\u8d28<\/strong><\/p>\n

\u6700\u8fd1\uff0c\u7855\u58eb\u751f\u5f20\u5bb6\u7487\uff08\u7537\uff09\u5728\u65b9\u771f\u6559\u6388<\/strong>\u7684\u6307\u5bfc\u4e0b\uff0c\u5728\u56fd\u9645\u5b66\u672f\u671f\u520aBioresource Technology \uff08Q1, IF 11.4\uff09\u53d1\u8868\u4e00\u7bc7\u5173\u4e8e\u4e24\u9636\u6bb5\u5171\u53d1\u9175\u6765\u81ea\u98df\u54c1\u5e9f\u5f03\u7269\u7684\u8461\u8404\u7cd6\u548cN<\/em>-\u4e59\u9170\u6c28\u57fa\u8461\u8404\u7cd6\u751f\u4ea7\u5fae\u751f\u7269\u6cb9\u8102\u7684\u7814\u7a76\u6027\u8bba\u6587\u3002<\/p>\n

\u4ee5Cryptococcus curvatus<\/em> \u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u901a\u8fc7\u5171\u53d1\u9175\u5927\u7c73\u6c34\u89e3\u7269\u548c\u867e\u58f3\u7684\u6c34\u89e3\u7269\uff0c\u5229\u7528\u4e24\u9636\u6bb5\u5de5\u827a\u5236\u5907\u5fae\u751f\u7269\u8102\u8d28\u3002\u8102\u8d28\u6837\u54c1\u7684\u4e3b\u8981\u6210\u5206\u662f\u78b3\u94fe\u957f\u5ea6\u4e3a16\u548c18\u7684\u957f\u94fe\u8102\u80aa\u9178\uff0c\u4e0e\u5178\u578b\u7684\u690d\u7269\u6cb9\u76f8\u4f3c\u3002\u8102\u8d28\u6837\u54c1\u663e\u793a\u51fa\u751f\u4ea7\u751f\u7269\u67f4\u6cb9\u7684\u8f83\u9ad8\u6f5c\u529b\u3002\u5728\u6447\u74f6\u53d1\u9175\u4e2d\uff0cN<\/em>-\u4e59\u9170\u6c28\u57fa\u8461\u8404\u7cd6\uff08GlcNAc\uff09\u548c\u8461\u8404\u7cd6\u7684\u5171\u540c\u5229\u7528\u63d0\u9ad8\u4e86\u7ec6\u80de\u8d28\u91cf\u548c\u8102\u8d28\u4ea7\u91cf\u3002\u5728\u7b2c\u4e00\u9636\u6bb5\u8fbe\u523017.60g\/L\u7684\u6700\u9ad8\u7ec6\u80de\u6d53\u5ea6\uff0c\u5728\u7b2c\u4e8c\u9636\u6bb5\u8fbe\u52300.233g\/g\u7684\u6700\u9ad8\u8102\u8d28\u4ea7\u7387\u3002\u6269\u5927\u81f35-L\u751f\u7269\u53cd\u5e94\u5668\u53d1\u9175\uff0c\u53ef\u5c06\u8102\u8d28\u542b\u91cf\u63d0\u9ad8\u523060.07%\uff0c\u4ea7\u91cf\u4e3a0.233g\/g\u3002Cryptococcus curvatus\u5728\u5927\u7c73\u6c34\u89e3\u7269\u548c\u867e\u58f3\u6c34\u89e3\u7269\u7684\u6df7\u5408\u7269\u4e2d\u57f9\u517b\u65f6\uff0c\u8102\u8d28\u542b\u91cf\u548c\u4ea7\u91cf\u5206\u522b\u4e3a52.25%\u548c0.204g\/g\u3002\u5927\u7c73\u6c34\u89e3\u7269\u548c\u7532\u58f3\u7d20\u6c34\u89e3\u7269\u7684\u8054\u5408\u53d1\u9175\u662f\u56de\u6536\u98df\u54c1\u5783\u573e\u7684\u6709\u6548\u9014\u5f84\u3002<\/p>\n

\u7ed3\u679c\u53d1\u8868\u5728Bioresource Technology\uff1a<\/p>\n

JX Zhang, XL Liu, L Wang, Zhen Fang<\/strong>*, Two-stage process production of microbial lipid by co-fermentation of glucose and N-acetylglucosamine from food wastes with Cryptococcus curvatus<\/em>, Bioresource Technology, 387, 129685, https:\/\/doi.org\/10,1016\/j.biortech.2023.129685<\/a> (2023<\/strong>).<\/p>\n","protected":false},"excerpt":{"rendered":"

Two-stage process production of microbial lipid by co-f […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5142"}],"collection":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/comments?post=5142"}],"version-history":[{"count":2,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5142\/revisions"}],"predecessor-version":[{"id":5146,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/5142\/revisions\/5146"}],"wp:attachment":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/media?parent=5142"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/categories?post=5142"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/tags?post=5142"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}