{"id":3977,"date":"2020-06-01T19:08:13","date_gmt":"2020-06-01T11:08:13","guid":{"rendered":"http:\/\/woodrefinery.com\/zhenfang\/?p=3977"},"modified":"2020-10-01T20:18:27","modified_gmt":"2020-10-01T12:18:27","slug":"microbial-lipid-production-from-both-rice-straw-hydrolysates-and-recycled-pretreated-glycerol","status":"publish","type":"post","link":"https:\/\/woodrefinery.com\/zhenfang\/microbial-lipid-production-from-both-rice-straw-hydrolysates-and-recycled-pretreated-glycerol\/","title":{"rendered":"Microbial Lipid Production from Both Rice Straw Hydrolysates and Recycled Pretreated Glycerol"},"content":{"rendered":"<p style=\"text-align: justify;\"><strong>Microbial Lipid Production from Both Rice Straw Hydrolysates and Recycled Pretreated Glycerol<\/strong><\/p>\n<p style=\"text-align: justify;\">Recently, PhD student <strong>Mr. Song Tang<\/strong> supervised by <strong>Prof. Zhen FANG<\/strong> produced microbial lipid from both rice straw hydrolysates and recycled pretreated glycerol. First, lipid fermentation of glucose <em>via Cryptococcus curvatus<\/em> was optimized by response surface methodology. Variables were selected by Plackett\u2013Burman design, and optimized by central composite design, achieving 4.9 g\/L total lipid and 0.16 g\/g lipid yield, and increased further as glucose increased from 30 to 50 g\/L. It was found that lipid content rapidly decreased from 44.5% to 6.4% as lignin (0.5 g\/L) was added, which would inhibit lipid accumulation for hydrolysate and recycled glycerol. Secondly, these fermentation conditions were further used for rice straw hydrolysates. After glycerol-FeCl<sub><span style=\"font-size: small;\">3<\/span><\/sub> pretreatment (0.06 mol\/L FeCl<sub><span style=\"font-size: small;\">3<\/span><\/sub>, 150 \u00b0C and 20 min), 72% lignin of rice straw was removed with glucose yield increased by 2.4 times to 74.3% at 20% substrate loading and 3 FPU\/g dry substrate. Its hydrolysates were separated for lipid fermentation, producing high total lipid (8.8 g\/L) and lipid yield (0.17 g\/g). Finally, recycled glycerol reached the maximum total lipid of 7.2 g\/L and high lipid yield of 0.16 g\/g. Based on the calculation, 2.9 g total lipid would be produced from 1 g rice straw and the recycled glycerol, with a similar composition to soybean oil.<\/p>\n<p style=\"text-align: justify;\">The results were published:<\/p>\n<p style=\"text-align: justify;\">S Tang,\u00a0Q Dong, <strong>Zhen Fang*<\/strong>, WJ Cong, H Zhang, Microbial Lipid Production from Both Rice Straw Hydrolysates and Recycled Pretreated Glycerol,\u00a0<u>Bioresource Technology<\/u>, <strong>312<\/strong>, \u200b123580 (<strong>2020<\/strong>). https:\/\/doi.org\/10.1016\/j.biortech.2020.123580.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3978\" src=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2020\/06\/6Figs-BT.jpg\" alt=\"\" width=\"898\" height=\"424\" srcset=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2020\/06\/6Figs-BT.jpg 898w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2020\/06\/6Figs-BT-300x142.jpg 300w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2020\/06\/6Figs-BT-768x363.jpg 768w\" sizes=\"(max-width: 898px) 100vw, 898px\" \/><\/p>\n<p style=\"text-align: justify;\">Microbial lipid production from both rice straw hydrolysates and recycled pretreated glycerol\uff08\u6c34\u7a3b\u79f8\u79c6\u7ecf\u7518\u6cb9-\u6c2f\u5316\u94c1\u9884\u5904\u7406\u540e\uff0c\u9176\u6c34\u89e3\uff0c\u5c06\u79f8\u79c6\u6c34\u89e3\u4ea7\u7269\u548c\u9884\u5904\u7406\u6db2\u4e2d\u7eaf\u5316\u7684\u7518\u6cb9\u4f5c\u4e3a\u78b3\u6e90\uff0c\u901a\u8fc7\u5f2f\u66f2\u9690\u7403\u83cc\u9ad8\u6548\u751f\u4ea7\u6cb9\u8102\u3002\uff09<\/p>\n<p style=\"text-align: justify;\"><strong>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;<\/strong><\/p>\n<p style=\"text-align: justify;\"><strong>\u7a3b\u79c6\u548c\u5e9f\u5f03\u7518\u6cb9\u53d1\u9175\u751f\u4ea7\u5fae\u751f\u7269\u6cb9\u8102<\/strong><\/p>\n<p style=\"text-align: justify;\"><strong>\u00a0<\/strong>\u6700\u8fd1\uff0c\u535a\u58eb\u751f<strong>\u5510\u677e<\/strong>\uff08\u7537\uff09\u540c\u5b66\u5728\u65b9\u8001\u5e08\u7684\u6307\u5bfc\u4e0b\uff0c\u5229\u7528\u7a3b\u79c6\u6c34\u89e3\u6db2\u548c\u9884\u5904\u7406\u6db2\u4e2d\u7eaf\u5316\u7684\u7518\u6cb9\u53d1\u9175\u751f\u4ea7\u5fae\u751f\u7269\u6cb9\u8102\u3002\u9996\u5148\uff0c\u901a\u8fc7\u54cd\u5e94\u9762\u4f18\u5316\u5f2f\u66f2\u9690\u7403\u83cc\u53d1\u9175\u8461\u8404\u7cd6\u4ea7\u6cb9\u8102\uff0c\u901a\u8fc7Plackett-Burman\u5b9e\u9a8c\u7b5b\u9009\u51fa\u663e\u8457\u6027\u56e0\u7d20\uff0c\u8bbe\u8ba1\u4e2d\u5fc3\u7ec4\u5408\u5b9e\u9a8c\u4ee5\u4f18\u5316\u6cb9\u8102\u4ea7\u91cf\uff0c\u5728\u6700\u4f73\u53d1\u9175\u6761\u4ef6\uff0c\u603b\u6cb9\u8102\u91cf\u4e3a4.9 g\/L\uff0c\u6cb9\u8102\u4ea7\u7387\u8fbe\u52300.16 g\/g\uff0c\u5e76\u901a\u8fc7\u589e\u52a0\u8461\u8404\u7cd6\u6d53\u5ea6\u4ee5\u8fdb\u4e00\u6b65\u63d0\u9ad8\u4e86\u5176\u4ea7\u6cb9\u8102\u91cf\u3002\u4f46\u5728\u6dfb\u52a0\u6728\u8d28\u7d20(0.5 g\/L)\u540e\uff0c\u9175\u6bcd\u80de\u5185\u6cb9\u8102\u542b\u91cf\u4ece44.5%\u8fc5\u901f\u964d\u4f4e\u81f36.4%\uff0c\u8fd9\u8868\u660e\u6728\u8d28\u7d20\u4f1a\u6291\u5236\u80de\u5185\u6cb9\u8102\u7684\u79ef\u7d2f\u3002\u6b64\u540e\uff0c\u5728\u4f18\u5316\u7684\u53d1\u9175\u6761\u4ef6\u4e0b\uff0c\u5229\u7528\u7a3b\u79c6\u6c34\u89e3\u4ea7\u7269\u53d1\u9175\u4ea7\u5fae\u751f\u7269\u6cb9\u8102\u3002\u901a\u8fc7\u7518\u6cb9-\u6c2f\u5316\u94c1\u9884\u5904\u7406\uff08150 \u00b0C \u548c 20 min\uff09\uff0c\u7a3b\u79c6\u4e2d72%\u6728\u8d28\u7d20\u88ab\u53bb\u9664\uff0c\u9176\u89e3\u7387\u8f83\u672a\u5904\u7406\u524d\u63d0\u9ad8\u4e862.4\u500d\u3002\u57283 FPU\/g\u5e72\u57fa\u548c20%\u57fa\u8d28\u6d53\u5ea6\u6761\u4ef6\u4e0b\uff0c\u9176\u6c34\u89e372 h\u540e\uff0c\u9884\u5904\u7406\u540e\u7a3b\u79c6\u7684\u9176\u89e3\u7387\u9ad8\u8fbe74.3%\u3002\u57fa\u4e8e\u54cd\u5e94\u9762\u4f18\u5316\u7684\u6700\u4f73\u8461\u8404\u7cd6\u4ea7\u6cb9\u8102\u7684\u53d1\u9175\u6761\u4ef6\uff0c\u5728\u5c06\u5206\u79bb\u7684\u79f8\u79c6\u6c34\u89e3\u4ea7\u7269\u4f5c\u4e3a\u78b3\u6e90\uff0c\u4ea7\u51fa\u6cb9\u81028.8 g\/L\uff0c\u540c\u65f6\u6cb9\u8102\u4ea7\u7387\u4e5f\u8fbe\u5230\u4e860.17 g\/g\u3002\u6700\u540e\uff0c\u5229\u7528\u9884\u5904\u7406\u6db2\u4e2d\u7eaf\u5316\u7684\u7518\u6cb9\u53d1\u9175\u751f\u4ea7\u5fae\u751f\u7269\u6cb9\u8102\uff0c\u603b\u6cb9\u8102\u91cf\u6700\u9ad8\u8fbe\u52307.2 g\/L\uff0c\u5e76\u83b7\u5f97\u4e86\u9ad8\u6c34\u5e73\u7684\u6cb9\u8102\u4ea7\u7387(0.16 g\/g)\u3002\u901a\u8fc7\u7269\u6599\u8d28\u91cf\u5e73\u8861\u5206\u6790\uff0c1 g\u7a3b\u79c6\u53ca\u5176\u9884\u5904\u7406\u4e2d\u6240\u7528\u7518\u6cb9\u5c06\u4ea7\u51fa2\u200b\u200b.9 g\u6cb9\u8102\uff0c\u5176\u8102\u80aa\u9178\u7ec4\u6210\u4e5f\u76f8\u4f3c\u4e8e\u5927\u8c46\u6cb9\u3002<\/p>\n<p style=\"text-align: justify;\">\u7ed3\u679c\u53d1\u8868\u5728Bioresource Technology:<\/p>\n<p style=\"text-align: justify;\">S Tang, Q Dong, <strong>Zhen Fang*<\/strong>, WJ Cong, H Zhang, Microbial Lipid Production from Both Rice Straw Hydrolysates and Recycled Pretreated Glycerol, <u>Bioresource Technology<\/u>, <strong>312<\/strong>, \u200b123580 (<strong>2020<\/strong>)<strong>.<\/strong> <a href=\"https:\/\/doi.org\/10.1016\/j.biortech.2020.123580\">https:\/\/doi.org\/10.1016\/j.biortech.2020.123580<\/a>.<\/p>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Microbial Lipid Production from Both Rice Straw Hydroly [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3977"}],"collection":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/comments?post=3977"}],"version-history":[{"count":5,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3977\/revisions"}],"predecessor-version":[{"id":4187,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3977\/revisions\/4187"}],"wp:attachment":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/media?parent=3977"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/categories?post=3977"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/tags?post=3977"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}