{"id":3752,"date":"2019-09-18T23:00:10","date_gmt":"2019-09-18T15:00:10","guid":{"rendered":"http:\/\/woodrefinery.com\/zhenfang\/?p=3752"},"modified":"2020-09-12T17:44:38","modified_gmt":"2020-09-12T09:44:38","slug":"3752-2","status":"publish","type":"post","link":"https:\/\/woodrefinery.com\/zhenfang\/3752-2\/","title":{"rendered":"High-Concentrated Substrate Enzymatic Hydrolysis of Pretreated Rice Straw with Glycerol and Aluminum Chloride at Low Cellulase Loadings"},"content":{"rendered":"<p><strong>High-Concentrated Substrate Enzymatic Hydrolysis of Pretreated Rice Straw with Glycerol and Aluminum Chloride at Low Cellulase Loadings<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3753\" src=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2019\/09\/2019-9-18graphic-abstract.jpg\" alt=\"\" width=\"1222\" height=\"789\" srcset=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2019\/09\/2019-9-18graphic-abstract.jpg 1222w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2019\/09\/2019-9-18graphic-abstract-300x194.jpg 300w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2019\/09\/2019-9-18graphic-abstract-768x496.jpg 768w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2019\/09\/2019-9-18graphic-abstract-1024x661.jpg 1024w\" sizes=\"(max-width: 1222px) 100vw, 1222px\" \/><\/p>\n<p style=\"text-align: justify;\">Based on a central composite design, glycerol and AlCl<sub>3<\/sub> pretreated rice straw with 0.08 mol\/L AlCl<sub>3<\/sub> at 146.8 \u00b0C for 20 min, resulting in 83% delignification, 94% hemicellulose removal, and 92% cellulose recovery for a remarkable glucose yield of 65.7% at high solid loads (15%) and low cellulase loading (3.3 FPU\/g dry substrate) with adding Tween 80 (40 mg\/g substrate). (\u901a\u8fc7\u54cd\u5e94\u9762\u4f18\u5316\uff0c\u5728146.8 \u00b0C\u548c0.08 mol\/L AlCl<sub>3<\/sub>\u6761\u4ef6\u4e0b\uff0c\u6c34\u7a3b\u79f8\u79c6\u7ecf\u7518\u6cb9-\u6c2f\u5316\u94dd\u9884\u5904\u740620 min\u540e\uff0c\u4fdd\u755992%\u7684\u7ea4\u7ef4\u7d20\uff0c\u540c\u65f6\u53bb\u966483%\u7684\u6728\u8d28\u7d20\u548c94%\u7684\u534a\u7ea4\u7ef4\u7d20\uff1b3 FPU\/g\u5e72\u57fa\u8d28\u548c15%\u57fa\u8d28\u6d53\u5ea6\u6761\u4ef6\u4e0b\uff0c\u6dfb\u52a040 mg\/g \u5e72\u57fa Tween 80\uff0c\u9176\u6c34\u89e348 h\uff0c\u9884\u5904\u7406\u540e\u6c34\u7a3b\u79f8\u79c6\u7684\u9176\u89e3\u7387\u53ef\u8fbe\u523065.7%\u3002)<\/p>\n<p style=\"text-align: justify;\">Recently, PhD student Mr. <strong>Song Tang<\/strong> supervised by Prof. Zhen FANG developed a glycerol and aluminum chloride pretreatment for lignocellulosic biomass. Rice straw was pretreated with glycerol and AlCl<sub>3<\/sub> for enzymatic hydrolysis at low cellulase loadings. Based on a central composite design, 83% delignification, 94% hemicellulose removal, and 92% cellulose recovery (or 76% cellulose in solid residue) were achieved under the optimized pretreatment conditions (0.08 mol\/L AlCl<sub>3<\/sub> as catalyst at 146.8 \u00b0C for 20 min with 90% glycerol). During glycerol-AlCl<sub>3<\/sub> pretreatment, the lignin-carbohydrate complex was depolymerized, resulting in the complex and recalcitrant construction of straw effectively being destroyed. The enzyme adsorption ability of pretreated straw was 16.5 times that for the original sample. After pretreatment, glucose yield was increased by 2.4 times to 74% for 48 h. Moreover, concentrated solid (15%) with low cellulase loading (3.3 FPU\/g dry substrate) achieved 58.6% glucose yield, and further increased by 12% to 65.7% by adding Tween 80.<\/p>\n<p style=\"text-align: justify;\">The results were published:<\/p>\n<p style=\"text-align: justify;\">S Tang,\u00a0Q Dong, <strong>Zhen Fang*<\/strong>, WJ Cong, ZD Miao, High-Concentrated Substrate Enzymatic Hydrolysis of Pretreated Rice Straw with Glycerol and Aluminum Chloride at Low Cellulase Loadings,\u00a0Bioresource Technology, <strong>294<\/strong>, 122164 (<strong>2019<\/strong>).<\/p>\n<p style=\"text-align: justify;\"><strong>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;-<\/strong><\/p>\n<p style=\"text-align: justify;\"><strong>\u7518\u6cb9\u548c\u6c2f\u5316\u94dd\u9884\u5904\u7406\u6c34\u7a3b\u79f8\u79c6\u7528\u4e8e\u4f4e\u7ea4\u7ef4\u7d20\u9176\u8f7d\u91cf\u6761\u4ef6\u4e0b\u9ad8\u57fa\u8d28\u6d53\u5ea6\u9176\u6c34\u89e3<\/strong><\/p>\n<p style=\"text-align: justify;\">\u6700\u8fd1\uff0c\u535a\u58eb\u751f<strong>\u5510\u677e<\/strong>\uff08\u7537\uff09\u540c\u5b66\u5728\u65b9\u8001\u5e08\u7684\u6307\u5bfc\u4e0b\uff0c\u5f00\u53d1\u51fa\u4e00\u79cd\u5e94\u7528\u4e8e\u6728\u8d28\u7ea4\u7ef4\u7d20\u751f\u7269\u8d28\u7684\u7518\u6cb9-\u6c2f\u5316\u94dd\u9884\u5904\u7406\u6280\u672f\uff0c\u5176\u5bf9\u6728\u8d28\u7ea4\u7ef4\u7d20\u7ec4\u5206\u5206\u79bb\u5177\u6709\u6781\u597d\u7684\u9009\u62e9\u6027\u3002\u901a\u8fc7\u54cd\u5e94\u9762\u4f18\u5316\uff0c\u6c34\u7a3b\u79f8\u79c6\u5728146.8 \u00b0C\u548c0.08 mol\/L AlCl<sub>3<\/sub>\u6761\u4ef6\u4e0b\uff0c\u5904\u740620 min\u540e\uff0c92%\u7684\u7ea4\u7ef4\u7d20\u88ab\u4fdd\u7559\uff0c83%\u7684\u6728\u8d28\u7d20\u548c94%\u7684\u534a\u7ea4\u7ef4\u7d20\u88ab\u53bb\u9664\u3002\u540c\u65f6\uff0c\u56fa\u4f53\u6b8b\u6e23\u4e2d\u7ea4\u7ef4\u7d20\u542b\u91cf\u8fbe\u523076%\u3002\u5728\u7518\u6cb9-AlCl<sub>3<\/sub>\u9884\u5904\u7406\u8fc7\u7a0b\u4e2d\uff0c\u6728\u8d28\u7d20-\u78b3\u6c34\u5316\u5408\u7269\u5f62\u6210\u7684\u590d\u5408\u7269\u88ab\u89e3\u805a\uff0c\u5bfc\u81f4\u79f8\u79c6\u590d\u6742\u548c\u987d\u56fa\u7684\u7ed3\u6784\u88ab\u6709\u6548\u5730\u7834\u574f\u3002\u6c34\u7a3b\u79f8\u79c6\u7ecf\u9884\u5904\u7406\u540e\uff0c\u5bf9\u7ea4\u7ef4\u7d20\u9176\u7684\u5438\u9644\u80fd\u529b\u63d0\u9ad8\u4e8616.5\u500d\uff0c\u9176\u89e3\u7387\u8f83\u539f\u59cb\u6c34\u7a3b\u79f8\u79c6\u63d0\u9ad82.4\u500d\uff0c\u8fbe\u5230\u4e8674%\u3002\u6b64\u5916\uff0c\u57283 FPU\/g\u548c15%\u57fa\u8d28\u6d53\u5ea6\u6761\u4ef6\u4e0b\uff0c\u9176\u6c34\u89e348 h\uff0c\u9884\u5904\u7406\u540e\u6c34\u7a3b\u79f8\u79c6\u7684\u9176\u89e3\u7387\u8fbe\u523058.6%\uff0c\u4e14\u6dfb\u52a040 mg\/g \u5e72\u57fa Tween 80\u540e\uff0c\u63d0\u9ad8\u4e8612%\uff0c\u8fbe\u523065.7%\u3002<\/p>\n<p style=\"text-align: justify;\">\u7ed3\u679c\u53d1\u8868\u5728Bioresource Technology:<\/p>\n<p style=\"text-align: justify;\">S Tang, Q Dong, <strong>Zhen Fang*<\/strong>, WJ Cong, ZD Miao, High-Concentrated Substrate Enzymatic Hydrolysis of Pretreated Rice Straw with Glycerol and Aluminum Chloride at Low Cellulase Loadings, Bioresource Technology, <strong>294<\/strong>, 122164 (<strong>2019<\/strong>).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>High-Concentrated Substrate Enzymatic Hydrolysis of Pre [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3752"}],"collection":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/comments?post=3752"}],"version-history":[{"count":5,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3752\/revisions"}],"predecessor-version":[{"id":4072,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3752\/revisions\/4072"}],"wp:attachment":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/media?parent=3752"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/categories?post=3752"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/tags?post=3752"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}