{"id":3374,"date":"2018-03-16T19:35:34","date_gmt":"2018-03-16T11:35:34","guid":{"rendered":"http:\/\/woodrefinery.com\/zhenfang\/?p=3374"},"modified":"2018-04-02T08:52:58","modified_gmt":"2018-04-02T00:52:58","slug":"cellulase-immobilized-on-mesoporous-biochar-synthesized-by-ionothermal-carbonization-of-cellulose","status":"publish","type":"post","link":"https:\/\/woodrefinery.com\/zhenfang\/cellulase-immobilized-on-mesoporous-biochar-synthesized-by-ionothermal-carbonization-of-cellulose\/","title":{"rendered":"Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose"},"content":{"rendered":"<p><strong>Cellulase immobilized on mesoporous biochar synthesized by ionothermal <\/strong><strong>carbonization of cellulose<\/strong><\/p>\n<p style=\"text-align: justify;\">Recently, <strong>Mr. Chang-hui Zhu<\/strong> (a PhD student from Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences) supervised by <strong>Prof. Zhen FANG<\/strong> synthesized biochar via ionothermal carbonization and pyrolysis, and it was subsequent used to adsorb free cellulase physically for enzymatic hydrolysis.<\/p>\n<p style=\"text-align: justify;\">In order to recover free cellulase, a mesoporous biochar was synthesized from cellulose by ionic thermal and pyrolysis methods for the adsorption of free cellulase. Kinetics analysis showed that the affinity of the immobilized cellulase to carboxymethyl cellulose was better than that of free cellulase. The immobilized cellulase at different pH and temperatures was more stable than those of free cellulase. It was used to hydrolyze pretreated cellulose with [Bmim]Cl with total reducing sugar yield of 99.9%. The immobilized cellulase activity can still retain 74.8% after 5 cycles. Lastly, the immobilized cellulase can improve sugar production from the pretreated corncob for potential practical applications.<\/p>\n<p style=\"text-align: justify;\">Results were published in <strong>Cellulose<\/strong>: Chang-hui Zhu, Zhen Fang*, Tong-chao Su, and Qi-ying Liu, Cellulase Immobilized on Mesoporous Biochar Synthesized by Ionthermal Carbonization of Cellulose, <strong>Cellulose,<\/strong>\u00a0 <strong>25(4)<\/strong>, 2473-2485 (<strong>2018<\/strong>).<\/p>\n<p style=\"text-align: justify;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3375\" src=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2018\/03\/xx.jpg\" alt=\"\" width=\"500\" height=\"375\" srcset=\"https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2018\/03\/xx.jpg 1268w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2018\/03\/xx-300x225.jpg 300w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2018\/03\/xx-768x576.jpg 768w, https:\/\/woodrefinery.com\/zhenfang\/wp-content\/uploads\/2018\/03\/xx-1024x768.jpg 1024w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/p>\n<p style=\"text-align: justify;\">Mesoporous biochar was synthesized by ionothermal carbonization, and pyrolysis for the immobilization of cellulase to hydrolyze ionic liquid pretreated cellulose and corncob. During pretreated cellulose hydrolysis, total reducing sugar yield of 92.2 % was achieved with 74.8% enzyme activity remained after 5 cycles.\uff08\u901a\u8fc7\u79bb\u5b50\u70ed\u548c\u70ed\u89e3\u65b9\u6cd5\u5408\u6210\u4e86\u4ecb\u5b54\u751f\u7269\u70ad\uff0c\u8be5\u751f\u7269\u70ad\u7528\u4e8e\u56fa\u5b9a\u7ea4\u7ef4\u7d20\u9176\uff0c\u56fa\u5b9a\u5316\u7ea4\u7ef4\u7d20\u9176\u7528\u4e8e\u6c34\u89e3\u79bb\u5b50\u6db2\u4f53\u9884\u5904\u7406\u7684\u7ea4\u7ef4\u7d20\u4ee5\u53ca\u7389\u7c73\u82af\u3002\u5176\u4e2d\u5728\u9884\u5904\u7406\u7ea4\u7ef4\u7d20\u6c34\u89e3\uff0c\u603b\u8fd8\u539f\u7cd6\u4ea7\u7387\u53ef\u8fbe92.2%\uff0c\u56fa\u5b9a\u5316\u9176\u5faa\u73af\u4f7f\u75285\u6b21\u540e\uff0c\u6d3b\u6027\u4ecd\u4fdd\u755974.8%\uff09\u3002<\/p>\n<p style=\"text-align: justify;\">\u6700\u8fd1\uff0c\u6731\u957f\u8f89\u540c\u5b66\uff08\u4e2d\u56fd\u79d1\u5b66\u9662\u5e7f\u5dde\u80fd\u6e90\u7814\u7a76\u6240\u535a\u58eb\u7814\u7a76\u751f\uff09\u5728\u65b9\u8001\u5e08\u7684\u6307\u5bfc\u4e0b\uff0c\u901a\u8fc7\u79bb\u5b50\u70ed\u548c\u70ed\u89e3\u6cd5\u7531\u7ea4\u7ef4\u7d20\u5408\u6210\u751f\u7269\u70ad\u4ee5\u53ca\u540e\u7eed\u7269\u7406\u5438\u9644\u5236\u5907\u56fa\u5b9a\u5316\u7ea4\u7ef4\u7d20\u9176\uff0c\u5e76\u5c06\u5176\u5e94\u7528\u4e8e\u79bb\u5b50\u6db2\u4f53\u9884\u5904\u7406\u7ea4\u7ef4\u7d20\/\u751f\u7269\u8d28\u7684\u6c34\u89e3\u3002<br \/>\n\u4e3a\u4e86\u56de\u6536\u6e38\u79bb\u7ea4\u7ef4\u7d20\u9176\uff0c\u901a\u8fc7\u79bb\u5b50\u70ed\u548c\u70ed\u89e3\u4e24\u6b65\u6cd5\u7531\u7ea4\u7ef4\u7d20\u5408\u6210\u4e00\u79cd\u4ecb\u5b54\u751f\u7269\u70ad\u4ee5\u7528\u4e8e\u5438\u9644\u6e38\u79bb\u7ea4\u7ef4\u7d20\u9176\u3002\u52a8\u529b\u5b66\u5206\u6790\u8868\u660e\uff0c\u56fa\u5b9a\u5316\u7ea4\u7ef4\u7d20\u9176\u5bf9\u7fa7\u7532\u57fa\u7ea4\u7ef4\u7d20\u7684\u4eb2\u548c\u529b\u4f18\u4e8e\u6e38\u79bb\u7ea4\u7ef4\u7d20\u9176\u3002 \u4e0d\u540cpH\u548c\u6e29\u5ea6\u4e0b\u7684\u56fa\u5b9a\u5316\u7ea4\u7ef4\u7d20\u9176\u6bd4\u6e38\u79bb\u7ea4\u7ef4\u7d20\u9176\u66f4\u7a33\u5b9a\u3002\u8be5\u56fa\u5b9a\u5316\u9176\u5177\u6709\u8f83\u597d\u7684\u50ac\u5316\u9884\u5904\u7406\u7ea4\u7ef4\u7d20\u6c34\u89e3\u7684\u80fd\u529b\uff0c\u603b\u8fd8\u539f\u7cd6\u4ea7\u7387\u53ef\u8fbe92.2%\uff0c\u56fa\u5b9a\u5316\u9176\u5faa\u73af\u4f7f\u75285\u6b21\u540e\uff0c\u6d3b\u6027\u4ecd\u4fdd\u755974.8%\u3002\u6700\u540e\uff0c\u8be5\u56fa\u5b9a\u5316\u9176\u7ed3\u5408\u79bb\u5b50\u6db2\u4f53\u9884\u5904\u7406\u6280\u672f\uff0c\u5bf9\u6c34\u89e3\u751f\u7269\u8d28\u7ea4\u7ef4\u7d20\u8868\u73b0\u51fa\u4e00\u5b9a\u7684\u5e94\u7528\u524d\u666f\u3002<br \/>\n\u7ed3\u679c\u53d1\u8868\u5728Cellulose: Chang-hui Zhu, Zhen Fang*, Tong-chao Su, and Qi-ying Liu, Cellulase Immobilized on Mesoporous Biochar Synthesized by Ionthermal Carbonization of Cellulose, <strong>Cellulose<\/strong> <strong>25(4)<\/strong>, 2473-2485 (<strong>2018<\/strong>).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cellulase immobilized on mesoporous biochar synthesized [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3374"}],"collection":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/comments?post=3374"}],"version-history":[{"count":3,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3374\/revisions"}],"predecessor-version":[{"id":3393,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/posts\/3374\/revisions\/3393"}],"wp:attachment":[{"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/media?parent=3374"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/categories?post=3374"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/woodrefinery.com\/zhenfang\/wp-json\/wp\/v2\/tags?post=3374"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}